June 2008 – Page 5 – My WordPress Site

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Astrobiology Science News 2 June 2008

by Keith CowingJune 2, 2008

Bright field microscopic image corresponding to pTERF image showing control S. cerevisiae cox 2-60, and chimeras S. cerevisiae cox2-60-SynJEC3, S. cerevisiae cox2-60-SynBD2, S. cerevisiae cox2-60-SynBD3 and S. cerevisiae cox2-60-SynBD8. Marged image of bright field chimeric cells (at gray) and pTIRF (X100) microscopic images can be predicted using 561 nm laser for visualization of endosymbiont autofluorescence chlorophyl (in red Em.=653/95 nm) from photosynthetically enabled engineered Syn. No such signal was detected from control yeast cells. SP8 Fluorescent confocal microscopic (x63) image of control S. cerevisiae cox 2–60, and endosymbiontic chimera. Marged images: host cell was stained using Conn A-FITC (emission at 510–520 nm wavelength, in green) and cyanobacterial autofluorescence can detected at 616-650 nm wavelength range. The experiment was repeated three times independently with similar results. -- Nature

Polar / Alpine Expeditions

Memorial for Astronauts and Cosmonauts Atop Mt. Everest

by Keith CowingJune 2, 2008December 23, 2023